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From 2011.igem.org
Revision as of 16:08, 7 July 2011 by RebekkaBauer (Talk | contribs)
Rebekka Bauer
novel in vivo mutagenesis mechanism:
-construct target genomic DNA with really strong promoter with retrovirus recognition sequence (R U5 PBS etc to ensure that only this is reverse transcribed)
-transcribe into RNA using a crap RNA pol that introduces mutations
-reverse transcribe into DNA using an overexpressed reverse transcriptase (overexpressed to ensure that this happens before DNA is degraded)
-insert into genome via homologous recombination using an equivalent of infusion enzyme (maybe RecA?)
