"
Team:Paris Bettencourt/Experiment/T7 diff subt subt microfluidic
From 2011.igem.org
Nanotube-assisted diffusion of T7 polymerase in Microfluidics
Experimental Scheme
In order to test whether our Bacillus subtilis T7 emitters & receivers can form nanotubes when mixed, we mix them in microfluidic system modified from Jeff Hasty's recent paper [1]. We imaged two channels: one injected with an emitter strain marked by RFP and a receiver strain; the other channel filled with only the receiver strain. The receiver strain contains the T7 autoloop, which will when activated by T7 polymerase
References
- Entrainment of a population of synthetic genetic oscillators. Mondragón-Palomino, O., Danino, T., Selimkhanov, J., Tsimring, L. & Hasty, J. Science 333, 1315-1319 (2011).
