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Grenoble 2011, Mercuro-Coli iGEM

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Biobricks

Here you can find all our news and composite parts we send to the registry.

<groupparts>iGEM011 Grenoble</groupparts>

You can also find in the registry some of our intermediate constructions:

BBa_S04602	RBS-CinR, BBa_B0034+BBa_C0077
BBa_S04603	RBS-TetR, BBa_B0034+BBa_C0040
BBa_S04606	RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077
BBa_S04605	RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240
BBa_S04607	pLac-CinR, BBa_R0011+BBa_C0077
BBa_S04618	pLac-CinR, BBa_K545006+BBa_I13401

MerR-del (K545103) MerR (BBa_K346001) without RBS PCR gel Well 1: Positive control (pSB4K5) Well 2: Negative control (Without DNA) Wells 3, 4, 5, 6, 7: MerR-del (band expected at 740pb, obtained around 700-800pb) Confirmed by sequencing
pMerT-GFP (K545002) Original parts: K346002, E0240 PCR gel Well 1: MW Well 2: empty well Well 3 and 4: clonning failure Well 5: pMerT-GFP (expected at 1200pb obtained right above 1000pb) Confirmed by sequencing
pCin-CrtEBI (K545000) Original parts: R0077, K274100 PCR gel Wells 1, 2 and 3: pCin-CrtEBI (Expected at 3500pb, obtained between 3000 and 4000pb) Well 4: MW Confirmed by sequencing
pConst-RBS-CinR (K545004) Original parts: J23119, B0034 C0077 Restriction gel Wells 1 and 2: pConst-CinR(expected at 950pb) Wells 4 and 5: cloning failure Wells 3 and 6: empty wells plasmids were obtained for each well at the expected size,about 2000pb. Confirmed by sequencing
pLac-CinR (S04607) Original parts: R0011, E0240 Restriction gel pConst-GFP (K545001) Original parts: J23119, E0240 Restriction gel Well 1: pLac-CinR (expected at 950pb) Well 2: pConst-CinR (expected at 900pb) plasmids were obtained for each well at the expected size,about 2000pb. Confirmed by sequencing
RBS-CinR (S04602) Original parts: B0034, C0077 PCR gel Well 1: RBS-CinR (expected at 1000) Wells 2, 3 and 4: clonning failure Confirmed by sequencing
RBS-TetR (S04603) Original parts: B0034, C0040 PCR gel well 1: RBS-TetR (expected at 950pb) Confirmed by sequencing