From 2011.igem.org
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Notebook - September
Lab Notes - October
Week13
| Day |
Note |
| Sept.26th Monday |
•Miniprep: Rush-3,-4,-12
•Run the gel of digestion test results:
RFP+YFP+1K3-7,-16 |
•PCR amplify: Rush-3,-4,-12
•Sample and test the intensity of fluorescence once or twice an hour during the growth of E.coli |
|
| Sept.27th Tuesday |
•Ligate and transform:
RFP+YFP+CFP+1C3 RFP+YFP+1C3 |
•PCR amplify:20H
•Cut: Rush Bone,22B |
|
| Sept.28th Wednesday |
•Cultivate: DH5αwith Device CFP,RFP,YFP and without any device
•Ligate and purify:Rush+22B
•Cut and purify:
the product of the ligation |
•Run the gel: Digested Rush and 22B,Lig1
•Self-ligate:Digested Lig
•Colony PCR: RFP+YFP+CFP+1C3
•Cut and purify: RFP+YFP+1K3,12I |
|
| Sept.29th Thursday |
•Ligate and transform: RFP+YFP+1K3&1C3,
RFP+YFP&1C3
•Colony PCR:RFP+YFP+CFP+1C3
•Preserve the colony on the plate |
|
•Plate the ligation results: RFP+1C3,
RFP+YFP+CFP+1C3
Colony PCR:RFP+YFP+1C3
RFP+YFP+CFP+1C3
•Cultivate the above
|
|
| Sept.30th Friday |
|
| Oct.1st Saturday |
•Colony PCR: Failed
•MIniprep: 10I |
•Cut: 10I+22B,20J,1T3
•Ligate:10I+22B+1T3 |
•Cultivate:2I,2M |
|
| Oct.2nd Sunday |
•Transform the ligation results:
22B+10I(1T3),20J+10I(1T3)
•Miniprep and Preserve:2M,2I |
•Cut:fdhf,Vgb,2M,2I
•Purify:RFP+YFP+CFP+1C3 |
•Ligate and transform:
RFP+YFP+CFP&1A3k
fdhf+2M&1K3,Vgb+2I&1K3 |
|
Week14
| Day |
Note |
| Oct.3rd Monday |
|
| Oct.4th Tuesday |
•Cut:RBS+20J
•Cultivate:fdhf+2M-1,-2,-3,-4
Vgb+2I-1,-222B+10I-1,-2
•Ligate and purify:RBS+20J& 1C3 |
•Cut:RBS+20J
•Ligate:2M+20J&10I to 1K3
•Transform:2M+20J+10I,Vgb+1C3
•Cultivate:22B+10I-1,-2 |
•Miniprep and cut: fdhf+2M,Vgb+2I
•Cut:fdhf+2M,20H |
|
| Oct.5th Wednesday |
•Cultivate:Vgb+2I 1-6
•Ligate:fdhf+20H& 1K3 |
•Transform:fdhf+20H
•Cut:2M+20J |
|
| Oct.6th Thurday |
|
| Oct.7th Friday |
|
| Oct.8th Saturday |
•Miniprep and test:
Vgb,fdhf+20H+1K3 1-4 |
•Colony PCR |
|
| Oct.9th Sunday |
•Digestion test and run the gel to test the results:
20J+10I -1,-2,-4
22B+10I -1,-2,-3,-4 Vgb+1C3
Fdhf+2M+20H -1,-2,-3,-4 Vgb+2I -1,-2,-5 |
•Colony PCR:fdhf+2M+20H 5-14
•Cultivate:fdhf+2M+20H 5-14
•Cut:fdhf+2M,20H |
|
Week15
| Day |
Note |
| Oct.10th Monday |
•Ligate:Fdhf+2M&20H to 1C3
•Cultivate:16L+Device C |
•Cut:Vgb+2I,22B+10I
•Ligate:Vgb+2I&22B+10I to1C3 |
|
| Oct.11th Tuesday |
| •Cultivate:20H,22B+10I-1,20J+10I-1,-4 |
•Miniprep and cut:
fdhf+2M+20H+1K3 5-14 |
|
| Oct.12th Wednesday |
| •Miniprep: fdhf+20H-10,20H,22B+10I, 20J+10I-1,-4 fdhf+2I+20H, |
•Cut and run the gel: Vgb+2I-5,
22B+10I,20J+10I,1C3 backbone |
|
| Oct.13th Thursday |
| •Cut and run the gel to test the ligate results |
|
| Oct.14th Friday |
| •Regional Jamboree @ HKUST |
|
|
|
| Oct.15th Saturday |
| •Regional Jamboree @ HKUST |
|
|
|
| Oct.16th Sunday |
| •Regional Jamboree @ HKUST |
We are the Champion!!! |
|
|
Week16
| Day |
Note |
| Oct.17th Monday |
|
| Oct.18th Tuesday |
|
| Oct.19th Wednesday |
| •Transform: Kit Plate 1 18E&18I, Kit Plate 4 7K& 7M |
|
| Oct.20th Thursday |
•Transform: Kit Plate 4 7K
•Colony PCR and run the gel:23L 1-6 |
|
| Oct.21st Friday |
•Transform:7K
•Cultivate:7M,18I,18E,23L pSB1A3,1K3,1C3 backbone |
•Cut and test the resistance: 22M+10I
•Colony PCR:7M 1-7,18E 1-6,18I 1-6 |
|
| Oct. 22nd Saturday |
•Cultivate:pSB1C3-5,23L-1,-2,7M-1
•Cultivate:7K,10I,22M,18E,18I
•Colony PCR:18E 1-6,18I 1-6
•Test the reagent for PCR several times |
•Miniprep and digestion test: 7M,23L,1K3,1A3,1C3
•Digestion and run the gel: 7M,22M+10I-2,1K3 |
|
| Oct.23rd Sunday |
•Cut:7M,22M+10I
•Digestion test:18I,18E |
•Ligate and transform: 7M+22M+10I& 1K3 |
|
Week17
| Day |
Note |
| Oct.24th Monday |
•Transform: Kit Plate 4 8G,8I Kit Plate 1 1F
•Culture yeast & B. subtilis |
•Colony PCR and run the gel: 7M+22M+10I+1K3 1-25
•Cultivate:8G,1F,8I |
|
| Oct.25th Tuesday |
•Miniprep:7M+22M+10I+1K3
•Colony PCR:8I,8G,1F
•Cut:7M+22M+10I+1K3,1C3 backbone |
•Cultivate:7K
•Ligate:7M+22M+10I& 1C3
•Genome DNA extraction from yeast & B. subtilis
•PCR condition pre-test for alsS & Adh2 |
|
| Oct.26th Wednesday |
•Cultivate:8G,8I,1F,7M,18E,18I
•Transform:7M+22M+10I+1C3 7M+22M+10I+1K3-2
•Reserve:7M+22M+10I+1K3,8G,8I, 1F,7M,18E,18I |
•Miniprep:8G
•Cultivate:7M+22M+10I+1C3，2J
•Testify PCR results on gel
•Clean-up alsS PCR product
•Re-pre-test PCR condition for Adh2 |
|
| Oct.27th Thursday |
•PCR producing Adh2
•alsS ES double-digestion
•purification both |
|
| Oct.28th Friday |
|
| Oct. 29th Saturday |
|
| Oct.30th Sunday |
|
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