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Team:UPO-Sevilla/Foundational Advances/Biobrick Creator/Creating Biobrick
From 2011.igem.org
Creating a new BioBrick
How does it work?
At first time, you should to be sure you are in the tab named "Creating a New BioBrick".
There are three levels of BioBrick parts: parts, devices and systems.
Parts are the building blocks and encode basic biological functions
(such as encoding a certain protein, or providing a promoter to let RNA polymerase
bind and initiate transcription of downstream sequences.
You can choose what part type you want convert to BioBrick standard, you can tick between:
- Protein Sequence Coding
- DNA
- Characterized Sequence
Protein coding sequences encode the amino acid sequence of a particular protein. Note that some protein coding sequences only encode a protein domain or half a protein. Others encode a full-length protein from start codon to stop codon. Besides, you can choose a codon optimization depending on a specific organism.
DNA sequence with a known function which can't be automatically optimized due to possible functional losses.
DNA parts provide functionality to the DNA itself. DNA parts include cloning sites, scars, primer binding sites, spacers, recombination sites, conjugative tranfer elements, transposons, origami, and aptamers.
To use your own sequence, simply write or paste your raw DNA sequence in 5'-3' or upload your raw DNA sequence using TXT or FASTA format files. For FASTA format files, if your files have incorrect or missing characters, it has to be fixed beforehand. For multiFASTA files, this aplication only reads the first sequence For TXT files, aplication will read every character inside the file, so after it has to erase the information not useful.
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