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Team:ZJU-China
From 2011.igem.org
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Project
Rainbofilm is a stratified expression system in
biofilm, a self-organized module extensible for various needs.
Researchers found a vertical oxygen gradient establishes in the biofilm.
Such property allows us to use oxygen sensitive promoters to
artificially induce differentiated functions through the spatial
distribution of cells. Thus, the multi-step reaction can be processed
within the different layers of the biofilm.
The biofilm and its layered structure form spontaneously.
Also biofilm has the natural resistance to high levels of toxin. These
two properties render the Rainbofilm a convenient stable system for
bio-production and bio-sensor. The system can cater to different needs
simply by changing downstream genes. One possible application is ethanol
production. The cellulose is degraded to monose from the bottom to the
middle layer, and the ethanol is produced and secreted in the surface to
minimize the toxicity to the inner cells.
Project
Rainbofilm is a stratified expression system in
biofilm, a self-organized module extensible for various needs.
Researchers found a vertical oxygen gradient establishes in the biofilm.
Such property allows us to use oxygen sensitive promoters to
artificially induce differentiated functions through the spatial
distribution of cells. Thus, the multi-step reaction can be processed
within the different layers of the biofilm.
The biofilm and its layered structure form spontaneously.
Also biofilm has the natural resistance to high levels of toxin. These
two properties render the Rainbofilm a convenient stable system for
bio-production and bio-sensor. The system can cater to different needs
simply by changing downstream genes. One possible application is ethanol
production. The cellulose is degraded to monose from the bottom to the
middle layer, and the ethanol is produced and secreted in the surface to
minimize the toxicity to the inner cells.
Team
We are a young and happy team of 13 scientists and engineers from very diverse backgrounds.Although this is our first year working on a wet lab project and biofilm experiments are new to us, we're currently taking great efforts to build the whole system for experiments.Our team progress and more info will be frequently updated so that our iGEM experience could be shared as much as possible along the way. We truly welcome you to contact us for any cooperation or suggestion.
Sponsors
