Team:Grenoble/Projet/Biobricks

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Revision as of 13:53, 27 October 2011 by Cerevisiea (Talk | contribs)

Grenoble 2011, Mercuro-Coli iGEM


Our Biobricks:

  • Here you can find all our news and composites parts we send to the registry.

<groupparts>iGEM011 Grenoble</groupparts> 

              		
			

				
    
				
  • You can also find in the registry some of our constructions intermediates:
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		BBa_S04602
		
RBS-CinR, BBa_B0034+BBa_C0077 

	

	

		BBa_S04603
		
RBS-TetR, BBa_B0034+BBa_C0040 

	
  
	

		BBa_S04606
		
RBS-TetR-RBS-CinR, BBa_B0034+BBa_C0040+BBa_B0034+BBa_C0077

	

	

		BBa_S04605
		
RBS-TetR-RBS-GFP, BBa_B0034+BBa_C0077+BBa_E0240

	

	

		BBa_S04607
		
pLac-CinR, BBa_R0011+BBa_C0077

	
 
	

		BBa_S04618
		
pLac-CinR, BBa_K545006+BBa_I13401

MerR-del : K545103.
MerR (BBa_K346001) without RBS
PCR gel
Well 1: Positive control (pSB4K5)
Well 2: Negative control (Without DNA)
Well 3, 4, 5, 6, 7: MerR-del (expected at 740pb, obtain around 700-800pb)
Confirmed by sequencing

pMerT-GFP: K545002
Original parts: K346002, E0240
PCR gel
Well 1 and 2: Failed cloning
Well 3: pMerT-GFP (expected at1200pb obtain just over 1000pb)
Confirmed by sequencing

pCin-CrtEBI (K545000)
Originally: R0077, K274100
PCR gel
Expected at 3500pb, obtain between 3000 and 4000pb
Confirmed by sequencing

pConst-RBS-CinR (K545004)
Originally J23119, B0034 C0077
Restriction gel
Well 1, 2: failed cloning
Well 3, 4: pConst-CinR(expected at 950pb)
Confirmed by sequencing

pLac-CinR (S04607)
Originally R0011, E0240
pConst-GFP (K545001)
Originally J23119, E0240
Restriction gel
Well 1: pLac-CinR (expected at 950pb)
Well 2: pConst-CinR (expected at 900pb)
Confirmed by sequencing

RBS-CinR (S04602)
Originally B0034, C0077
PCR gel
Well 1: RBS-CinR (expected at 1000)
Confirmed by sequencing

RBS-TetR (S04603)
Originally B0034, C0040
PCR gel
RBS-TetR (expected at 950pb)
Confirmed by sequencing

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