"
Team:Peking S/project/wire/matrix
From 2011.igem.org
Template:Https://2011.igem.org/Team:Peking S/bannerhidden Template:Https://2011.igem.org/Team:Peking S/back2
Template:Https://2011.igem.org/Team:Peking S/bannerhidden
Chemical Wire Toolbox
Introduction|Harvesting ‘Chemical Wires’ From Nature|Synthesizing Quorum Sensing Inverters|Orthogonal Activating Matrix
Orthogonal activating matrix
Figure 1 A graphical representation of the activation associated with five receiving systems and their corresponding inducer.The concentration of salicylate is 10^-4 mol/L. LuxI product and RhlI product is the 10 fold dilution of the supernatant from a overnight culture bearing pT7 controlled luxI generator and ptet controlled rhlI generator, respectively. The concentration of 3-oxododecyl-homoserine lactone(3OC12HSL) is 10^-8 mol/L, and the CinI product is a 1:100 dilution of the overnight culture broth.
Table 1 Original Data of the activating matrix, showing absolute GFP fluorescence(a.u.)
Table 2 Data of the activating matrix, average fold compared with blank samples calculated
Table 3 Data of the activating matrix, activating fold normalized
</center>
