Team:Peking S/project/wire/matrix

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Chemical Wire Toolbox


Introduction|Harvesting ‘Chemical Wires’ From Nature|Synthesizing Quorum Sensing Inverters|Orthogonal Activating Matrix


Orthogonal activating matrix


Aiming at effective and discrete communications in a multicellular genetic network, orthogonality of the 'chemical wires' is essential. Thus we carried out a series of experiments checking the signaling orthogonality of 'chemical wires' in our toolbox.

SignalingOrthogonal.png

Figure 1 A graphical representation of the relative activation fold associated with five receiver systems and their corresponding inducer.The concentration of salicylate is 10^-4 mol/L. LuxI product and RhlI product is 10 fold dilution of the supernatant from a overnight culture bearing pT7 controlled luxI generator and ptet controlled rhlI generator, respectively. The concentration of 3-oxododecyl-homoserine lactone(3OC12HSL) is 10^-8 mol/L, and the CinI product is 1:100 dilution of the overnight culture broth.

Table 1 Original Data of the activating matrix, showing absolute GFP fluorescence(a.u.)

SR Matrix table1.png


Table 2 Data of the activating matrix, average fold compared with blank samples calculated

SR Matrix table2.png


Table 3 Data of the activating matrix, activating fold normalized

SR Matrix table3.png


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