Team:Minnesota/leex4462-04162011000200-28

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== Restriction Digest of pucBB and ompA inserts == Project: Surface Display Date: Sat, 16 Apr 2011 00:02:00 GMT Author: Sukdong Lee (leex4462) Access: Public Revision History:
  • Mon, 22 Aug 2011 10:00:33 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose: Redo the digest of both insert and vector. Procedure -Use NdeI and NotI with NEB Buffer 3. -Add 0.4 uL of BSA to prevent degradation or star activity (most likely due to NdeI). -Check the products after 1-2 hours to see if degradation occurred.
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