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Team:UNAM-Genomics Mexico/Project/RhizobialKit
From 2011.igem.org
Contents |
General Overview
Abstract
Expanding our ability to manipulate and engineer biological parts in new organisms is an ever present goal of synthetic biology. With this idea in mind we set out to develop a kit of biological tools to enable working with BioBricks in Rhizobial species. We developed the tools in this kit in our model chasis is Rhizobium etli, a member of the Rhizobiaceae family of nitrogen fixing bacteria, that establishes a symbiotic relationship with legumes within the root nodules.
Rhizobial Kit Description
The Rhizobial Kit will set the ground for the use of Rhizobial species, especially R. etli, as organisms available for the design and construction of novel synthetic biological machines. The kit will be composed of parts with distinct molecular functions that will increase the repertoire of possible design and engineering approaches in Rhizobial species. The parts that we will develop are the following:
- The nifH promoter. This promoter sequence comes from the promoter region of the nifHa gene in R. etli. It functions in conditions of low oxygen concentration. (¿también en condiciones de bajo nitrógeno fijado? referencia). This promoter region comprises a contains a σ54-dependent promoter located between position -24 and -12 relative to the transcription start site, as well as a binding site for NifA, a positive transcriptional regulator (Beat Thöny, Hauke Hennecke, 1989; Valderrama, B., et. al., 1996).
