Project Abstract

Ionizing radiation and radiation pollution is an important environmental problem that not only affects those working around radiation facilities, but those dealing with the aftermath of widespread nuclear disasters such as those at the Fukushima Daiichi nuclear reactor. Penn State’s team project designed and constructed a genetic circuit introduced into E. coli bacterial cells, in order to rapidly detect and report the presence of harmful ionizing radiation. We are working to create a robust and reliable biosensor that utilizes the lambda phage lytic-lysogenic switch as the radiation sensor. When the sensor detects radiation, it triggers one of three fast acting reporters we developed based on the concept developed by Imperial College’s 2010 iGEM team will be triggered. Each of the reporters features a different enzyme/substrate reaction (β-galactosodise/β-D-galactose, β-glucurodinase/β-D-glucuronide or C23O/catechol). We believe that the final construct may have the potential to rival current radiation detection methods, such as digital dosimeters.

Team

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Ben Aloudir

Junior
Biotechnology and Microbiology

Brian Avison

Junior
Engineering Science

Alex Bina

Junior
Bioengineering

Alex wanted to join iGEM because I wanted to design and construct the genetically engineered spider that transformed Peter Parker into Spiderman. When he found out that was impossible, I used every opportunity to sabotage the Penn State iGEM team. No one has heard from Alex since August. Last we heard, he was moonlighting as State College's dark knight.

Jamie Colleta

Junior
Biological Engineering

Anisha Katyal

Anisha is a senior majoring in biology. She heard about iGEM through a friend and thought it would be a great research experience. When she is not in the lab, she is actively involved in the biology club.

Elyse Merkel

Elyse is a senior majoring in Engineeing Science. She has always had an interest in genetic engineering and iGEM provided her with a unique opportunity to pursue this interest. When not in the lab, Elyse spends her time participating in all things Penn State, including Relay For Life of Penn State, THON committees, and Lion Ambassadors.

Byron Pierce

Senior
Chemical Engineering

Jim Rose

Jim is a senior majoring in biotechnology. He joined the iGEM team because it is "freaking awesome". Jim spends his time outside of iGEM doing research in the biochemistry department on the JC virus, and getting stuck in freezers.

Lauren Rossi

Lauren is a senior majoring in Microbiology with a minor in Spanish and Biology and Molecular Biology. She was a participant of the Penn State iGEM team in 2010 and through this experience she developed a interest in synthetic biology. After attending the Jamboree last year, she knew she wanted to participate in iGEM again. When she is not in the lab, Lauren spends her time as a TA for a microbiology lab and is also the Vice President for the American Society for Microbiology.

Vishal Saini

Vishal is a senior double majoring in Science and Psychology with a minor in Neuroscience. He wanted the chance to get a unique research experience and thought iGEM would offer him this opportunity. Vishal spends his time outside the lab serving as the THON Chair for OPENN State and helping students as undergraduate Chemistry Tutor. He is also a Resident Advisor and an active member of the Global Medical Brigades.

Kristen Salava

Kristen is a junior majoring in bioengineering. She heard about iGEM from one of her bioengineering professors and thought it would be a great way to learn more about what synthetic biology can do. Outside of the lab, she spends most of her time with her sorority where she is the Vice President of Standards. She also is an International Envoy within the college of engineering.

Swati Prasad

SWATI PUT STUFF HERE!!!!!!!!!!!

Mike Speer

Graduate Student

Dr.Tom Richard

Advisor
Professor of Agricultural and Biological Engineering

Dr. Howard Salis

Advisor
Assistant Professor of Agricultural Engineering

Brainstorming

In the first few days of summer, we began discussing ideas for our project. Some of our first initial thoughts were to modify bacterial photosynthesis using the BCHM gene or to engineer a serotonin sensor. We thought about making a bacterial fuel cell by using Geobacter as a sacrificial anode. We also liked the idea of using bacteria to perform intelligent tasks, like mathematical arithmetic. The last idea we developed was to engineer a bacteria that would be able to detect radiation. The team spent a few days researching these topics to determine how plausible they would be and we finally unanimously agreed upon the bacterial radiation detector. With our full thoughts focused on this topic, we began looking into this idea further and discovered that the Penn State 2007 iGEM team had used the lambda phage system to also develop a bacteria that would test for radiation. However, they were not able to fully assemble their device or test it and we thought that we would be able to build upon their initial foundation.

Research

After deciding to follow up on the bacterial dosimeter idea for our project, we as a team we had to first do some research on the effects of ionizing radiation in order to better determine what exactly we would be detecting.

Currently all living organisms are exposed to a relatively low dose of radiation; this amount is so negligible that the negative effects are unnoticeable. The problem with ionizing radiation occurs when one is exposed to a high dose for a short period and/or low doses over an extended period of time. The International Commission on Radiological Protection (ICRP) recommends nuclear industry employees to limit ones yearly exposer to 50 mSv, or 100 mSv over 5 years.¹ One international study, composing 15 countries and encompassing 598,068 nuclear industry workers, found that an increased risk of cancer exists. Specifically, 99% of those tested were exposed to less than 50 mSv of radiation per year, and 1-2% of these cancer related deaths may be attributable to this exposer. It’s also estimated that 100 mSv of cumulative radiation exposer would result to a 5.9% increase chance mortality.²

1. Cardis, E. "Risk of Cancer after Low Doses of Ionising Radiation: Retrospective Cohort Study in 15 Countries." Bmj 331.7508 (2005): 77. Print.
2. Shapiro, B. "1990 Recommendations of the International Commission on Radiological Protection." European Journal of Radiology 15.1 (1992): 93. Print.