Team:Harvard/Lambda Red

Lambda Red

Lambda red recombineering makes use of homologous recombination systems to allow the insertion of constructs into the genome. It is accomplished in two steps, as shown in the diagram below and in our Protocols section. Also see Lambda Red results for how we used lambda red to build our selection system.

Figure 1. Lambda Red, PCR to get the required insertion product (zeocin in this example) 915px