Wildtype
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
0, .0014, .0028, .0042, .0056, .0070, .0140
0.99315,
0.78019,
0.58405,
0.45400,
0.35401,
0.29099,
0.13305,
4
0, 1, 2, 5
0.00000, 0.20000, 0.40000, 0.60000, 0.80000, 1.00000, 2.00000
0.99315, 1.02097, 0.99023, 0.99354,
0.78019, 0.97021, 0.96725, 0.96226,
0.58405, 0.87100, 0.89795, 0.94529,
0.45400, 0.76333, 0.85071, 0.93618,
0.35401, 0.65243, 0.75444, 0.88056,
0.29099, 0.55454, 0.68266, 0.84441,
0.13305, 0.26829, 0.37015, 0.55294
Mutant 1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
0, .0014, .0028, .0042, .0056, .0070, .0140
0.68829,
0.48655,
0.27198,
0.15260,
0.08426,
0.05322,
0.01741,
Mutant 2
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattattgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca
0, .0014, .0028, .0042, .0056, .0070, .0140
0.68514,
0.43727,
0.27724,
0.17817,
0.11684,
0.08021,
0.03415,
Mutant 3
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaaagtgagttagctcactcattaggcaccccaggctttacactttaagcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
0, .0014, .0028, .0042, .0056, .0070, .0140
0.67026,
0.44322,
0.28504,
0.17543,
0.10938,
0.07325,
0.02937,
Mutant 4
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgcgcgcaacgcaactaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacgca
0, .0014, .0028, .0042, .0056, .0070, .0140
0.31888,
0.16625,
0.10228,
0.05821,
0.03985,
0.02549,
0.01329,
Mutant 5
caatacgcaaaccgcctctccccgcgcgtcggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctccctcatcaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
0, .0014, .0028, .0042, .0056, .0070, .0140
0.63174,
0.33524,
0.17440,
0.09337,
0.03043,
0.03337,
0.01686,
Mutant 6
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctctacactttatgcttccggcttgtatgttgtgtggaattgtgagcggataacaatttcacaca
0, .0014, .0028, .0042, .0056, .0070, .0140
0.42290,
0.29347,
0.15733,
0.09471,
0.05778,
0.03850,
0.01475,
Mutant 7
caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca
0, .0014, .0028, .0042, .0056, .0070, .0140
0.58989,
0.32402,
0.20047,
0.11183,
0.06365,
0.03915,
0.01712,
"
Some preliminary data. These are LacI promoter mutants. The green bars represent mutants that are outside of 1.5 standard deviations of wildtype LacI promoter. There's still more to come!
