From 2011.igem.org

Purified Enzyme Assay

• Assay
  • Add 90uL of 5uM substrate (in NaAc pH 4.0 buffer) to each well of a black fluorescent microtiter assay plate
  • Start reaction by adding 0.0125mg/mL enzyme (try to avoid bubbles and pippette quickly, but accurately)
    • Use the P20 multichannel with a taped cardboard stopper to make sure you don't hit the pellet!
  • Monitor the reaction with the SpectraMax