Team:Washington/Magnetosomes/Background

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iGEM Toolkits

As with the expansion of the iGEM competition, many iGEM teams have started to investigate the possibility to make multiple-gene insert for their project as to bring the cloning project from single gene level to multiple gene level. However, current Biobrick Standard vectors are not designed for multiple-insert cloning. Thus, UW IGEM team decided to research on methods to improve the cloning efficiency and two "toolkits" were brought to the Register as a result.


Gibson Assembly Toolkit

As a continuation of 2010 UW IGEM project, this year we created and submitted several plasmid backbones that are Gibson cloning method friendly aka pGA vectors. It is called the Gibson Assembly Toolkit


Igem2011 GibsonToolkit.png

What's in the Gibson Assembly Toolkit?

  • Five plasmid backbones
  • pGA1A3, pGA1C3, pGA3K3, pGA4A5, pGA4C5









Magnetosome Toolkit

In addition, we were also ambitious about assembling a large gene-construct of over 16 kb. Therefore, utilizing our pGA vectors and Gibson cloning methods, the Magnetosome Toolkit was developed with the goal to build magnetic E.Coli; a novel characteristic expressed solely by magnetotactic bacteria, such as Magnetospirillum magneticum strain AMB-1


Igem2011 MagnetToolkit.png

What’s in the Magnetosome Toolkit?

  • A set of the 18 essential genes for the various steps in magnetosome formation.
  • Our favorite genes in pGA vectors
  • A table compiling individual gene functions from our literature search
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