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Team:EPF-Lausanne/Our Project/Data
From 2011.igem.org
Data
team, please check this page: [1]
Contents |
Favourite Parts
These are officially our three favourite parts:
[http://partsregistry.org/Part:BBa_K613015 BBa_K613015]
[http://partsregistry.org/Part:BBa_K613016 BBa_K613016]
[http://partsregistry.org/Part:BBa_K613017 BBa_K613017]
Other New parts
T7 Promoter
Our lysis selection device uses what we refer to as the wild-type T7 Promoter:
[http://partsregistry.org/Part:BBa_K613001 K613001]
In addition, we submitted the following mutants:
Variants without additional lac operator (constitutive):
[http://partsregistry.org/Part:BBa_K613002 K613002]
[http://partsregistry.org/Part:BBa_K613003 K613003]
[http://partsregistry.org/Part:BBa_K613004 K613004]
[http://partsregistry.org/Part:BBa_K613005 K613005]
[http://partsregistry.org/Part:BBa_K613006 K613006]
Variants with additional lac operator (LacI repressed):
[http://partsregistry.org/Part:BBa_K613007 K613007]
[http://partsregistry.org/Part:BBa_K613008 K613008]
[http://partsregistry.org/Part:BBa_K613009 K613009]
[http://partsregistry.org/Part:BBa_K613010 K613010]
[http://partsregistry.org/Part:BBa_K613011 K613011]
[http://partsregistry.org/Part:BBa_K613012 K613012]
TetR mutants
In-vivo testing was used to characterise these:
- V36F mutant: [http://partsregistry.org/Part:BBa_K613013 K613013]
- V36F W43S double mutant: [http://partsregistry.org/Part:BBa_K613014 K613014]
- E37A W43S T141A triple mutant: [http://partsregistry.org/Part:BBa_K613015 K613015]
- P39K mutant: [http://partsregistry.org/Part:BBa_K613016 K613016]
- Y42F K108E double mutant: [http://partsregistry.org/Part:BBa_K613018 K613018]
- P39Q Y42M double mutant: [http://partsregistry.org/Part:BBa_K613019 K613019]
Medium-strength Plac
[http://partsregistry.org/Part:BBa_K613000 K613000]
TetR Mutants
| TetR variants | Mutagenesis | Promoter | Cterminal | DNA form | Expression | MITOMI vs 1-off | Biobrick | Sequenced | Sent to registry |
|---|---|---|---|---|---|---|---|---|---|
| E37AW43ST141A | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613015 | v | v |
| P39K | PCR-induced | T7 | His-tag | template | ITT tested, worked | v | BBa_K613016 | v | v |
| Y42F | PCR-induced | T7 | His-tag | linear | ITT tested | v | BBa_K613017 | v | v |
| Y42FK108E | PCR-induced | T7 | His-tag | linear | ITT tested | BBa_K613018 | v | v |
T7 promoter variants
Pre-existing parts we improved
- [http://partsregistry.org/Part:BBa_K112808:Experience Experience] - The Berkeley Lysis Device, BBa_K112808: In developing our Lysis selection device, we characterised induction, and ran proof-of-principle experiments.
