Team:Freiburg/Notebook/21 June

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Sequencing

Primer concentration has to be 2 nM.
Dilution of stock: 2 μl in 100 μl H2O.
max 100 ng/μl of DNA.


pos. clone ng/μl μl
PR1e 46.8 2.1
PR2d 67.9 1.4
PR3e 45.8 2.2
PR4b 61.5 1.6
PR5a 74.5 1.3
PR6c 94.7 1.06
PR7b 69.1 1.4
PR8b 81.9 1.2
PR9a 64.4 1.5
lys 1 16 10
lys 2 21.7 10
lys 3 2.5 10
lys 4 16.4 10
lys 5 10.2 10