Microfluidic system
We have modified a microfluidic system from Jeff Hasty group in UCSD, in which we can grow bacterial cells continuously for a long time in a single layer. We called it the
Micro-chemostat. Compared to the usual microcolony-on-agar-pad method in the paper, this system will be able to maintain exponential growth of the bacterial cells in close contact with each other for a long time without exhausting the nutrient nor form double layers. We hope by extending the imaging time, we'll be able to observe the more diffusion-through-nanotube events in a single experiment.
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