Team:Groningen/project notebook/18 July 2011

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March M T W T F S S   [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_March_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]	April M T W T F S S         [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_April_2011 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_April_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/17_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_April_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_April_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_April_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]	May M T W T F S S             [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_May_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_May_2011 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_May_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_May_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_May_2011 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_May_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_May_2011 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_May_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_May_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_May_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_May_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_May_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_May_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_May_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_May_2011 20] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/21_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_May_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_May_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_May_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_May_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_May_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_May_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_May_2011 30] [http://2011.igem.org/Team:Groningen/project_notebook/31_May_2011 31]	June M T W T F S S     [http://2011.igem.org/Team:Groningen/project_notebook/1_June_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_June_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_June_2011 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_June_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_June_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_June_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_June_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_June_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_June_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_June_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_June_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_June_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_June_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_June_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_June_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_June_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_June_2011 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_June_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_June_2011 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_June_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_June_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_June_2011 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_June_2011 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_June_2011 30]
July M T W T F S S         [http://2011.igem.org/Team:Groningen/project_notebook/1_July_2011 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_July_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_July_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_July_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_July_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_July_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_July_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_July_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_July_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_July_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_July_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_July_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_July_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_July_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_July_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_July_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_July_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_July_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_July_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_July_2011 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_July_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_July_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]	August M T W T F S S [http://2011.igem.org/Team:Groningen/project_notebook/1_August_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_August_2011 2] [http://2011.igem.org/Team:Groningen/project_notebook/3_August_2011 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_August_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_August_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_August_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_August_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_August_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_August_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_August_2011 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_August_2011 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_August_2011 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_August_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_August_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_August_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_August_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_August_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_August_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_August_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_August_2011 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_August_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_August_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_August_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_August_2011 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_August_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/Team:Groningen/project_notebook/29_August_2011 29] [http://2011.igem.org/Team:Groningen/project_notebook/30_August_2011 30] [http://2011.igem.org/Team:Groningen/project_notebook/31_August_2011 31]	September M T W T F S S       [http://2011.igem.org/Team:Groningen/project_notebook/1_September_2011 1] [http://2011.igem.org/Team:Groningen/project_notebook/2_September_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/Team:Groningen/project_notebook/4_September_2011 4] [http://2011.igem.org/Team:Groningen/project_notebook/5_September_2011 5] [http://2011.igem.org/Team:Groningen/project_notebook/6_September_2011 6] [http://2011.igem.org/Team:Groningen/project_notebook/7_September_2011 7] [http://2011.igem.org/Team:Groningen/project_notebook/8_September_2011 8] [http://2011.igem.org/Team:Groningen/project_notebook/9_September_2011 9] [http://2011.igem.org/Team:Groningen/project_notebook/10_September_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/11_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 11] [http://2011.igem.org/Team:Groningen/project_notebook/12_September_2011 12] [http://2011.igem.org/Team:Groningen/project_notebook/13_September_2011 13] [http://2011.igem.org/Team:Groningen/project_notebook/14_September_2011 14] [http://2011.igem.org/Team:Groningen/project_notebook/15_September_2011 15] [http://2011.igem.org/Team:Groningen/project_notebook/16_September_2011 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_September_2011 17] [http://2011.igem.org/Team:Groningen/project_notebook/18_September_2011 18] [http://2011.igem.org/Team:Groningen/project_notebook/19_September_2011 19] [http://2011.igem.org/Team:Groningen/project_notebook/20_September_2011 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_September_2011 21] [http://2011.igem.org/Team:Groningen/project_notebook/22_September_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/23_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/24_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/25_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/26_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/27_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/28_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_September_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30]	October M T W T F S S           [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/1_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/2_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/3_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/4_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/5_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/6_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/7_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/8_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/9_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/10_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 10] [http://2011.igem.org/Team:Groningen/project_notebook/11_October_2011 11] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/12_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/13_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/14_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/15_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/16_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 16] [http://2011.igem.org/Team:Groningen/project_notebook/17_October_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/18_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/19_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/20_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 20] [http://2011.igem.org/Team:Groningen/project_notebook/21_October_2011 21] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/22_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 22] [http://2011.igem.org/Team:Groningen/project_notebook/23_October_2011 23] [http://2011.igem.org/Team:Groningen/project_notebook/24_October_2011 24] [http://2011.igem.org/Team:Groningen/project_notebook/25_October_2011 25] [http://2011.igem.org/Team:Groningen/project_notebook/26_October_2011 26] [http://2011.igem.org/Team:Groningen/project_notebook/27_October_2011 27] [http://2011.igem.org/Team:Groningen/project_notebook/28_October_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/29_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/30_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:Groningen/project_notebook/31_October_2011&preload=Team:Groningen/project_notebook/Day&action=edit 31]

Mon 18 July 2011

Vessa

To Do List

• Wet lab stuff preparation
• Design primer --> for inverting RFP+DT construct
• Meeting with Prof. Oscar Kuipers after lunch

Joyce
Today, Digestion, DNA clean up with High Pure PCR purification kit of Roche, ligation, transformation to create transformants
with: PBAD-LasR-LVA-DT and PhybB-LasR-LVA-DT
Also, colony of PBAD-RBS-GFP and PBAD-cI-LVA-DT were grown overnight in LB medium with ampicillin and this morning, the culture had been grown, so plasmid prep, glycerol stock and sample for sequencing should be made.
Digestion:
LasR-LVA-DT vector
2μl vector
1μl EcoR1
1μl XbaI
3μl Fast digest buffer
1μl Fast AP
22μl MQ

pBAD
10μl pBAD
1μl EcoR1
1μl SpeI
2μl Fast digest buffer
6μl MQ

PhybB
3μl PhybB
1μl EcoR1
1μl SpeI
2μl Fast digest buffer
13μl MQ
Incubate the samples at 37°C for 30 min.
Do a DNA purification with the PCR purification kit from Roche
Ligation:

PBAD-LasR-LVA-DT vector
8.5μl vector
6.1μl insert
1μl T4 DNA ligase
2μl T4 DNA ligase buffer
2.4μl MQ

PhybB-LasR-LVA-DT vector
8.5μl vector
4.1μl insert
1μl T4 DNA ligase
2μl T4 DNA ligase buffer
4.4μl MQ

Self ligation:
8.5μl vector
1μl T4 DNA ligase
2μl T4 DNA ligase buffer
8.5μl MQ

Ligate at room temperature for 30 min.

Transform as usual (see 12th of July)

Plasmid prep of overnight culture construct: LasR-LVA-DT (plasmid backbone with ampicillin resistance)
Elute in 100μl MQ
Use 10μl for gel electrophoresis to check wether a plasmid is present in the sample. Plasmid was seen on a 1% agarose gel with
TBE (with the help of gelred)
Nanodrop result: 67.2 ng/μl.
Send for sequencing: 5μl Sample + 5μl primer of 5μM.
Glycerol stock was made: 250μl Glycerol 85% + 750μl bacterial culture (overnight culture)

Colony PCR with samples PBAD-cI-LVA-DT, PhybB-cI-LVA-DT and PBAD-RBS-GFP-DT (samples ligation mixtures stored overnight in fridge)was done as followed:
Mastermix scheme:
Taq 10× buffer with NH4SO4 without MgCl2: 58.00μl
dNTPs 10mM each: 11.6μl
MgCl2: 34.8μl
Taq polymerase 5u/μl: 2.90μl
Biobrick vector forward primer 10μM: 11.6μl
Biobrick vector reverse primer 10μM: 11.6μl
MilliQ water: 449.50μl

PCR conditions:
Preheated lid: 111°C
Denaturation: 94°C for 10 min.
Cycle (33×)
Denaturation: 94°C for 30s.
Annealing: 60°C for 30s.
Extension: 72°C for 2 min.
Final extension: 72°C for 10 min.
Store at 4°C infinite

PCR products were analysed on a 1% agarose gel with TBE.
Results: PBAD-cI-LVA-DT colony 1 seems to be right (correct size on gel (2kb) )
PhybB-cI-LVA-DT can be right, is difficult to see, maybe use one colony and tomorrow (with other colony PCR probably, pick
some of the old plates of this sample aswell.
PBAD-RBS-GFP-DT colony 4 looks alright, but is difficult to see because the gel is not very good, so just try along with the
pBAD-cI-LVA no right transformants, but the one of friday will be used as the sample send for sequencing.

PCR for PhybB PCR product (there is not much left anymore...)

PCR was done as followed:
Mastermix scheme:
Taq 10× buffer with NH4SO4 without MgCl2: 25.00μl
dNTPs 10mM each: 5μl
MgCl2: 15μl
Taq polymerase 5u/μl: 1.25μl
Pfu polymerase: 0.2μl
Forward primer PhybB: 5μl
Reverse primer PhybB: 5μl
MilliQ water: 193.55μl
End volume in PCR tube: 50μl.

PCR conditions:
Preheated lid: 111°C
Denaturation: 94°C for 10 min.
Cycle (37×)
Denaturation: 94°C for 30s.
Annealing: 65°C and 70°C (gradient) for 30s.
Extension: 72°C for 2 min.
Final extension: 72°C for 10 min.
Store at 4°C infinite (overnight)

Jakub
Week 29:

• Cloning LasR variants into plasmids with reverse double terminator and LasB promoter - second try.