Team:Groningen/project characterisation mu lasr
From 2011.igem.org
LasR Autoinducing Loops
Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.
Leakiness of the LasR autoinducing loops
no-tag | DAS | LVA | ||
RBS 0,07 | | | | |
RBS 0,3 | | | | |
RBS 0,6 | | | | |
RBS 1 | | | | |
Figure 4. Flow cytometer data taken at different time-points during exponential growth of cells carrying different cI autoinducing loops. |