Team:TU Munich/project/data

Data For Our Favorite New Parts

BBa_K568003 - T7 promoter with lacZ reporter part:

Our original reporter construct. The presence of a functional T7 polymerase causes expression of beta-galactosidase. Thus, it is possible to detect the output of our optogenetical AND-Gate.

BBa_K568002 - blue light promoter with lacZ reporter part:

The blue light promoter followed by lacZ is a simple optogenetic device that expresses beta-galactosidase if the blue light input is positive. We used this part to find out the characteristics of the response to blue light in ''E. coli''. The blue light promoter is part of the YcgE/F system which is a blue light sensor that is naturally found in ''E. coli''. Thus, only the promoter itself is needed in this part.

BBa_K568001 - optogenetical AND-Gate red/blue light:

This part is the centerpiece of our 3D-printer. It is supposed to cause expression of a functional T7 polymerase only when both the red light and the blue light input are positive. Further characterization will be needed.

All submitted parts

BBa_K568000 - red light sensor:

BBa_K568001 - optogenetical AND-Gate red/blue light:

BBa_K568002 - blue light promoter with lacZ reporter part:

BBa_K568003 - T7 promoter with lacZ reporter part:

BBa_K568004 - optogenetical AND-Gate withouth first promoter - choose your first input:

BBa_K568005 - Intermediate optogenetical AND-Gate withouth T7ptag:

BBa_K568006 - Intermediate synthetic part:

Data For Pre-existing Parts

Experience - hopcyA, BBa_K322127 (Edinburgh, 2010):

Experience - T7 promoter driving 6-his tagged P7 GFP, BBa_I746907 (Cambridge, 2007):

We used this as additional reporter plasmid and did an IPTG induced GFP assay to verify the part. It worked perfectly for us.