Team:EPF-Lausanne/Our Project/T7 promoter variants/t7make/variants

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Introduction
Our Project
Tools
- Gibson Assembly
- MITOMI
Notebook
- Protocols
- May
- June
- July
- August
- September
- October
Considerations
- Human Practices
- Safety
Acknowledgements
- Sponsors
- Partners

The List of T7 Promoter Variants

Strength	Mutation	Biobrick Sequence
Wildtype	None	TAATACGACTCACTATAGGGAGA
.14	-16	TGATACGACTCACTATAGGGAGA
.3	-2	TAATACGACTCACTACAGGGAGA
.54	-17	CAATACGACTCACTATAGGGAGA
.8	3	TAATACGACTCACTATAGGGTGA
1.11	3	TAATACGACTCACTATAGGGCGA

The Different T7-Lac Promoter Variants

Strength	Mutation	Biobrick Sequence
Wildtype	None	TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAATT
.14	-16	TGATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAATT
.3	-2	TAATACGACTCACTACAGGGAGAGGAATTGTGAGCGGATAACAATT
.54	-17	CAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAATT
.8	3	TAATACGACTCACTATAGGGTGAGGAATTGTGAGCGGATAACAATT
1.11	3	TAATACGACTCACTATAGGGCGAGGAATTGTGAGCGGATAACAATT

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