pBAD/AraC promoter

In our system and measurements we have been using the pBAD/AraC promoter ([http://partsregistry.org/Part:BBa_I0500 BBa_I0500]). This promoter is functioning in our circuit as a jammer for the LasR autoinducing loop. It is also used to induce the cI autoinducing loops during the measurements. That is why, in our opinion, characterizing this promoter in a way that would show us the activity rate not only depending on concentration of arabinose, but also in a dynamic way - over time - is crucial. The measurements were done as described in protocol.

Below you will find four figures representing the results of our measurements.

Experiment 1a. Cells carrying a pSB1C3 plasmid with pBAD/AraC-GFP-DT construct, induced at time=0h.

Experiment 1b. Wild type cells, induced at time=0h.

Experiment 2a. Cells carrying a pSB1C3 plasmid with pBAD/AraC-GFP-DT construct, induced at time=0h.

Experiment 2b. Wild type cells, induced at time=0h.