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Team:UCL London/Research/Stresslights
From 2011.igem.org
The Concept of Stresslights 2.0
The development of a bioprocess for the production of a useful biological product on a commercial scale involves extensive optimisation procedure in order to maximise the yield. The idea of stress lights 2.0 has been extended from the UCL iGEM 2009 project in order to develop a toolkit with a collection of useful and robust sensory device for the industry. The basic theme is to identify particular promoter sequences which can be used to detect a particular stress or stimuli relevant to bioprocess development and ligate it to a fluorescent protein expression cassette, in order to monitor that particular stress in real-time in a quick, efficient and simple way. The major modification this year was the addition of a LVA tag for faster degradation of the fluorescent proteins in order to reduce their half life and increase the accuracy and sensitivity of the stress readings.
