Team:Osaka/week7

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Revision as of 09:18, 16 September 2011 by Toshi (Talk | contribs)


Contents

September 11(Sun)

  1. Gel extraction of digests
  2. Miniprep of yesterday's culture
  3. Restriction digests of minipreppped parts
  4. Gel electrophoresis of digests
  5. Ligation
    • 103(C):PprM+1-3A(vector)
    • 104(C):RecA+1-3A(vector)
    • 105(A):12(upstream)+101(downstream)+1-1G(vector)
    • 106(A):12(upstream)+102(downstream)+1-1G(vector)
  6. Transformation of ligation products


September 12(Mon)

  1. To liquid culture:103,104,105,106

September 13(Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Ligation
    • 107(A):12(upstream)+103(downstream)+1-1G(vector)
    • 108(A):12(upstream)+104(downstream)+1-1G(vector)
  5. Transformation of ligation products

September 14(Wed)

  1. To liquid culture:107,108

September 15(Thu)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:108

September 16(Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests


September 17(Sat)

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