From 2011.igem.org

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PCR Conditions and Primers of Registry Parts

Please fill out for every PCR reaction done on a registry part (both new and old).

LEGEND

• Amplification Success (S, M, N): Was the amplification successful.
  • S = Single Band at Desired Size
  • M = Multiple Bands, but one at desired length
  • N = No band at desired length
• Template Part #: The Registry Number of the Part being amplified from
• Template Form: The source form of the template DNA
  • Miniprep (M)
  • Colony (C)
  • PCR Product (P)
• Template Source: Where the template is originally from, such as 2011 Parts Kits, Synthesized Gene, Genomic, etc
• Forward Primer (5'->3'): Full sequence of the Forward primer used
• Reverse Primer (5'->3'): Full sequence of the Reverse primer used
• Annealing Temperature (Celsius): The annealing temperature used in the PCR reaction
• Extension Time (seconds): The extension time used in the PCR reaction
• Percent DMSO: Was DMSO added, if not 0, if so at what %
• Polymerase Used: Which polymerase enzyme was used (e.g. Phusion, Taq, Vent, Pfu, etc)
• Primer Name: The name of the primer ordered
• Additional Notes: Any additional relevant information

Tool

[http://excel2wiki.net/index.php Excel to wiki format tool]

Recreate the table exactly in excel and fill in with data

Copy the all the data (no header) into the box

Copy the code starting with the line that looks like |- do not copy header stop copying at |- as well

Paste into this page's code

• there is a big comment where you should paste the code

EXAMPLE TABLE (DO NOT MODIFY)