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Team:Paris Bettencourt/Experiments/YFP TetR diffusion
From 2011.igem.org
Experiments of the YFP concentration design
The planning of the experiments is the following : first we have tested the strains from D.Lane containing YFP:tetR and tetO array. Then we constructed/biobricked the YFP:tetR and tetO array system. To finish with the microscopy step and results of this proof of concept between B. subtilis and B. subtilis / E. coli.
Testing the YFP:tetR strains from D. Lane
Biobricked system construction
Biobricked system construction
In the emittor cell (B. Subtilis), we have inserted a expressive system for the YFP:tetR. It contains the promoter pVeg, the RBS for B. Subtilis and the YFP:tetR protein. Production of YFP:tetR will diffuse throught the nanotube to the receiver cell.
In the receiver cell (B. Subtilis or E. Coli), there is the tetO array where diffused YFP:tetR will concentrate. The YFP is the monitor of the signal.
The principle of the design is summed up in the image below
Fig1: Schematics of the YFP concentration design
