Team:Paris Liliane Bettencourt/Notebook/2011/09/05/

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Cyrille

Miniprep

Miniprep of 2 clones of TetO array in pSB1C3, 2 clones of TetO array in pHM3 Miniprep of Mathias: S21 S82 1 2 3 4; S75 S67 and S75

With good yeilds (>500ng)

Gel Extraction

Gel extraction of pHM3 Digested in EP and pVeg YFP TetR D EP and DXP.

Ligation

Ligation of pHM3 Digested EP with pVeg-YFP-TetR D. EP with ratio 1:1 1:10 1:100 Ligation pHM3 TetO D. SP with pVeg-YFP-TetR D. SP

Transformation

Transformation of the ligation product + ComS ligated in pSB1C3 + YFP TetR BB ligated in pSB1C3

Contol of the comptent cells

Two tests of transformation was done with the B7 plasmid in TurboCell competent cells.

The rest of the tube is placed in 500µL of LB, incubated for an hour and then plated.

Hovannes-Baptiste

Preparation of slides

Dilution of overnight cultures : YC164 and YC227 (SinOp Design) .

Two well slides : 1-control (YC164) 2-Mix (both)

Observation

-37°C Microscopy-

YC164 grows fast
YC227 does not grow at all
YC164 does not change its state
We can see some GFP after an overnight

No significant results