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Data

TetR Mutants

• V36F
• P39K
• Y42F
• P39Q-Y42M

T7 Promoter Plasmids

• pSB3K1-T7-const-RFP
• pSB3K1-T7-lac-RFP
• pSB3K1-T7-lac2-RFP
• pSB3K1-T7-const-Lysis
• pSB3K1-T7-lac-Lysis

• pSB3C5-T7-const-RFP
• pSB3C5-T7-lac-RFP
• pSB3C5-T7-lac2-RFP
• pSB3C5-T7-lac-Lysis
• pSB3C5-T7-lac2-Lysis

T7 Promoter Variants

Reporter Plasmids

J61002 Ptet-RFP

TetR plasmids

pSB3K1 Pconst-TetR

Parts assembled:

• Plasmid backbone: pSB3K1 from ETHZ 2007 [http://partsregistry.org/wiki/index.php?title=Part:pSB3K1] (taken from the delivery plate)
• Pconst: J23116 from Berkeley 2006 [http://partsregistry.org/Part:BBa_J23116] (sequence copied into our primers)
• RBS and spacer: (sequence copied into our primers)
• TetR: from Alina?

Sequencing results:

Sequenced data compared to the sequence of Pconst,RBS+spacer and TetR gene: File:EPFL pSb3K1 TetR seq.txt All these parts are correct.

pSB3K1 Pconst-TetR Ptet-LacI

Parts assembled

• Plasmid backbone containing Pconst and TetR: see precedent section
• Terminator: B0014 from the Registry [http://partsregistry.org/Part:BBa_B0014] (sequence copied into our primers)
• Ptet: R004 from Registry [http://partsregistry.org/Part:BBa_R0040] (sequence copied into our primers)
• LacI: amplified from Repressilator plasmid

Sequencing results: