Team:Freiburg/Notebook/29 August

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green light receptor

PCR to amplify Green light Promotor PcpcG

Investigators:Julia

Testdigest of quickchanged CcaR

Investigators:Julia,with help of the team

blue light receptor

3A-assembly

Investigators: Sandra

Digestion I:

• Lovtap
• Not-Gate
• pSB1A3
• pSB1T3

stored in the blue light box.

Ligation

• ♥-A3 Not
• ♥-A3 nOt
• ♥-A3 noT
• ♥-T3 Not
• ♥-T3 nOt
• ♥-T3 noT

stored in the ligation box.

Digestion II:

Also digestion with new amplified vector

• pSB1C3

Lovtap was digested again this time also with DpnI. So for this ligation the ♥-PCR3A, digested with DpnI, and pSB1C3 and Not, nOt, noT were used.

• ♥-C3 Not
• ♥-C3 nOt
• ♥-C3 noT

Transformation

Investigators: Sophie

transformation of:

• ♥-A3 Not
• ♥-A3 nOt
• ♥-A3 noT
• ♥-T3 Not
• ♥-T3 nOt
• ♥-T3 noT

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME

Precipitator

NAME OF YOUR EXPERIMENT

Investigators: NAME