Team:Paris Liliane Bettencourt/Notebook/2011/07/05/

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ligation and transformation

Axel

ligation

• BBa_k143079 6.4ng/µL in BBa_K090403 (vector) 9.2ng/µL
• BBa_K145001 8.5ng/µL - BBa_B0015 (in the vector) 10.9ng/µL

Babak

Gel purification

• well 1: ladder 100pb
• from well 2: BBa_B1010 cut by EcoR1 and S, concentration of DNA=8,9ng/µL

Digestion

• BBa_K090403 cut by EcoR1 and XbaI
• BBa_SB1C3,2070pb, cut by EcoR1 and Ptsx
• BBa_J18913,2428pb, cut by EcoR1 and XbaI
• BBa_pHyperspank,121pb, cut by Xbal and Ptsx
• B4 pVeg + SpoVg,117pb, cut by EcoR1 and SpeI

Mathias & Laura

After sequencing, we could see the BioBrick the parts registry sent us under the name K143033 was not LacI but a sorbitol so we have to redo the construction the parts LacI+ double terminator.