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  • Gibson Assembly
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Todo

Microfluidic Chips

• Continue alignment training

Preparing the parts

We need to sequence-verify the lysis brick and LacI.

Status: Primers for sequencing the lysis brick have been ordered; reaction solutions for LacI sequencing have been prepared (ready to send out?).

Next steps:

• Make competent cells
• Transform in E. Coli.
• Glycerol Stocks
• Plasmid preps
• Sequence the lysis cassette

Assembly

• Research plasmid backbones. Two different types are needed.
• Design Gibson primers to assemble the three different plasmids
• Determine which sequence on plasmids "two" and "three" should be used to create the "mega-plasmid"

Wiki

• Start uploading protocols
• Write-up team presentation
• Activate Javascript accordeon menu
• Fix alignement

Clean rooms

• Order lab notebook
• Order storage box