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Team:Northwestern/Notebook/Protocols/Overnight
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PROJECT
RESULTS
CONSIDERATIONS
ABOUT US
NOTEBOOK
ATTRIBUTIONS
Overnight
- Take 5mL LB and put in a round bottom tube
- If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube
*MAKE SURE YOU ADD THE RIGHT ONE!
- Take a tip on a p200 and lightly touch a colony
- Eject the tip into the culture and put the cap on
- For a miniprep, grow O/N for 12-16 hours NOT LONGER
Adapted from [http://openwetware.org/wiki/Bacterial_cell_culture OpenWetWare]
REMEMBER TO USE PROPER STERILE TECHNIQUE
