Team:Freiburg/Notebook/8 June

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Protein modelling and amplification of parts

Investigators:
Protein modelling with pymol: Rüdiger
Amplification of different parts: Julia and Jacob

antibiotics plates:
1oo mg/ml Ampizilin (100 microliter per plate)
1oo mikroliter H2O + 2 microliter Cm
1oo mikroliter H2O + 40 microliter Tet
1oo mikroliter H2O + 10 microliter Kan

part location(p=plate) resistance info
BBa_K098995 P3, 1E Amp heat sensitive cI QPI with high promoter
BBa_K112022 P3, 24E amp Lambda phage lysis device - no promoter, This part is in BBb Format.
It is flanked by BamHI and BglII sites instead of XbaI and SpeI.