Team:IIT Madras/Dry lab/Modelling/Simulations

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Simulations for Proof of Concept




Figure 1 and 2 : % Increase in growth rate observed due to Proteorhodopsin (Proton efflux = 2 and 10 mmol/g-dw hr) for varying glucose uptake rates respectively.



According to the model the following reactions showed major flux changes due to Proteorhodopsin :

  1. 'adentylate kinase GTP '
  2. 'adenosine hydrolase'
  3. 'dihydroorotic acid menaquinone 8
  4. '3 isopropylmalate dehydrogenase'
  5. 'psicoselysine transport via proton symport periplasm
  6. 'purine nucleoside phosphorylase Deoxyadenosine '
  7. 'L threonine via sodium symport periplasm '
Reaction Knockout Analysis of '3 isopropylmalate dehydrogenase' was lethal and the other reactions didn’t have major effects on growth rates .



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