-Speeding Up-

Purification of HNS(wt)and electrophoresis and purification of pSB1C3. Although both concentrations were not enough, try to ligate and transformation HNS(wt) and pSB1C3.

-Visualization-

-Colony PCR

Samples which transformed one day ago.

-Electrophoresis

Above samples

-Preculture

Promoter

Suppressed expression system

RFP

@plasmid extraction, DNA refine, DNA densitometry, restriction emzyme(EcoRⅠ, SpeⅠ), electrophoresis OriTr-GFP

@preculture from transformed 5th Aug.