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Team:KIT-Kyoto/Project
From 2011.igem.org
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The fireworks using AHL as a transmitter.
The bacteria communicate with each other in the same way as people do so using some languages.This was called Quorum Sensing, and we paid our attention to this Quorum Sensing.In Quorum Sensing, the bacteria use the chemical substance called "autoinducer" in substitution for words used by a human being.The bacteria confirm how much density the same type of bacteria exists by around oneself with autoinducers.When they sense the density is high, the bacteria take action all at once. We plan to produce bioart using Quorum Sensing as materials this time.E. coli uses a chemical substance called "N-Acyl-Homoserin-Lacton(AHL)" as autoinducers.We thought whether we could reproduce fireworks by bioart using AHL as a communication factor.We use the GFP to express flower firelight.The GFP,which is green fluorescence protein discovered in Aequorea victoria, has the property of forming a chromophoric group (chemical structure to develop a color) alone. In short, fireworks will be launched and turned off by the following methods.
① GPF is expressed in E.coli using AHL as a communication factor ② Turn off the expressed GPF like a chain reaction.
