Team:UTP-Panama/Week 10

Week 10: August 8 to 13

August 8

WET LAB

Place: INDICASAT
Work Session #36 (morning)
Transformation of Competents Cells with pSB1A3, for ensure that our creation of competents cells work.

OBJECTIVE: Preparation of culture medium. 1. JM 109 strain used for cloning plasmids and growth. This is a commercial strain that comes in a kit, were planted in a solid LB medium and then treated to make them competent.

August 9

WET LAB

Place: INDICASAT
Work Session #37 (morning)
The Transformation works!!! Preparation of 4 falcons tube with trasnformed cells (for growth). (Objetives or Title): --ESCRIBIR Y MEJORAR LO QUE VA-- escribir que plasmido era del registry

August 10

Work Session #38 (morning)
Place: INDICASAT Project Design Group: session of selection & analisys of the Gatech and Bristol Biobricks models and funtioning. We started to study the possibility of use the CspA promoter to improve gene expression under cold shock stress.
Human Practice: preparation of concepts and definitions to the outreach campaign.

WET LAB

Work Session #39 (morning)
Minipred of our Transformed Cells.
(Objetives or Title): --ESCRIBIR Y MEJORAR LAB---
Work Session #40 (afternoon)
--ESCRIBIR--
Finishing Miniprep, preparation of Plasmid for electrophoresis. Left running the Electrophoresis.

August 11

WET LAB

Work Session # (Objetives or Title): --ESCRIBIR Y MEJORAR LAB--

Human Practice

Preparation of brochures for Outreach. Preparation of the slides regarding to the presentation for Outreach. Mindstorm about SynBio definitions & implications.

August 12

WET LAB

Work Session # (Objetives or Title):

Human Practices
We were studying about the pros and cons of the SynBio and the safety regulations (for example the Synthetic Biology Report of the Presidential Commission for the Study of Bioethical Issues) for explain the risks and the safety to the people.