Team:Arizona State/Lab/Protocols/PCR

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PCR:

Prepare master mix (for 8 tubes):
10*n uL 5x Phusion buffer
4*n uL 2.5 mM dNTPs
2.5*n uL forward primer
2.5*n uL reverse primer
29.5*n uL PCR water
Add 48 uL master mix to each of seven tubes.
Label tubes blank, 0, 1, 2, 4, 8, 10
Add DNA and MgCl2 to tubes according to chart:

Blank 0 1 2 4 8 10
MgCl2 0 0 .5 1 2 4 5
DNA 0 1 1 1 1 1 1

Add 0.5 uL Phusion polymerase to each tube.
Place tubes in thermocycler.
Adjust settings as needed.

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