Week 2:
06/13/2011
1. Lab meeting
06/14/2011
1. Order the primer for PCR
06/15/2011
1. DNA isolation
2. PCR genes we need
06/16/2011
1. DNA isolation for E.Coli strains DH5 α, HT115, BL21
2. PCR
06/17/2011
1. Run a gel to test the genes we made yesterday
Observation: all genes for Yeast and CRISPR project worked
2. Removal of single stranded Extension For:
pSB1A3, pSb1K3, OLE1, MSN2, TSP1, CAS0
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