From 2011.igem.org

Gibson Purification

• Prepare this mixture on ice to prevent the reaction from beginning early

1. Add 5 volumes of buffer PB to 1 volume of the Gibson product.
2. Pour/Pipet the mixture into a spin column (pink).
3. Centrifuge the sample for ~ 1 minute. Discard the flow-through.
4. Wash the sample by adding 750 μL buffer PE and centrifuge for ~ 1 minute.
5. Centrifuge the sample for ~ 1 minute. Discard the flow-through.
6. To Elute the DNA, place the spin column in a clean 1.5 μL (labeled) microcentrifuge. Add 30 μL of buffer EB and let Stand for 1 minute!
7. Centrifuge the sample for ~ 1 minute.
8. Proceed to the Transformation (Electroporation) protocol.