Team:EPF-Lausanne/Todo
From 2011.igem.org
Todo
Contents |
Microfluidic Chips
- Continue alignment training
- Run experiments on "chemostat" chip to check design
- Adapt design of "chemostat" chip for e-coli
Preparing the parts
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Sequence the lysis cassette - Double-check lysis cassette sequence
Assembly
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Research plasmid backbones. Is there one that already contains Tet-repressed LacI or GFP? - Design Gibson primers to assemble the three different plasmids.
- Determine which sequence on LacI-plasmid and Lysis-plasmid should be used to create the "mega-plasmid".
Wiki
Protocols
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Describe cell cultures in miniprep protocol - Write a new protocol!
General
- Write-up team presentation
- Upload our initial research about transcription factors
Clean rooms
- Order lab notebook
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Order storage box