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muTetRs

In vitro Main | Why TetR? | Mutant TetRs | MITOMI Data | In-vivo & In-vitro outline

Why

How

What

TetR variants	Method	Promoter	C-terminal	DNA form	Expression	MITOMI vs 1-off	Biobrick	Sequenced	Sent to registry
V36F	site directed mutagenesis	SP6	eGFP	plasmid	worked well	v	BBa_K613013
V36FW43S	PCR-induced mutagenesis	T7	His-tag	linear	(ins C-term), ITT tested		BBa_K613014
E37AW43ST141A ||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||v||BBa_K613015||v||v |- | |||||||||||||||||| |- | |||||||||||||||||| |- | P39K ||PCR-induced mutagenesis||T7||His-tag||template ||ITT tested and worked ok||v||BBa_K613016||v||v |- | |||||||||||||||||| |- | Y42F||PCR-induced mutagenesis||T7||His-tag||linear||ITT tested||v||BBa_K613017||v||v |- | Y42FK108E||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613018||v||v |- | |||||||||||||||||| |- | P39QY42M ||site directed mutagenesis||SP6||eGFP||plasmid||worked well||v||BBa_K613019|||| |- | P39QY42ML197S||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613020|||| |- | P39QY42ML52P||PCR-induced mutagenesis||T7||His-tag||linear ||ITT tested||||BBa_K613021|||| |- | |||||||||||||||||| |- | E37AP39K||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613022||v|| |- | |||||||||||||||||| |- | E37AP39QY42F||site directed mutagenesis||T7||His-tag||linear ||||||BBa_K613023 ||v|| |} {{:Team:EPF-Lausanne/Templates/Footer}}