Team:EPF-Lausanne/Tools/MITOMI
From 2011.igem.org
MITOMI
Mitomi is a high troughput microfluidic system designed to mesure low affinity molecular interactions by mechanically trapping them.
Purpose: We applied this technology during our project for characterizing transcription factor binding energy landscapes in absolute terms by determining dissociation constants (Kd) for a comprehensive set of target DNA sequences.
Schematic of the Mitomi platform:
The control inputs control the valves that close the Detailed view of the chambers and of the movable membrane used for mechanical trapping, referred to as the 'Button' .
Advantages: The movable memrane shown in the scheme below enables measurements of transient interactions, exhibiting sub micromolar affinities which are generally problematic.
Running a MITOMI experiment
MITOMI scans explanation