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Team:Groningen/project characterisation mu
From 2011.igem.org
Memory Units
cI Autoinducing Loops
The autoinducing loop driven by cI will function as our first memory unit. We aimed to find a combination of RBS, transcription factor and degradation tag that enables us to toggle between the two steady states in a robust way. To estimate the rate in which single cells are activated by too much leakage of cI protein we measured the fluorescence in uninduced cells with a flow cytometer. To measure the activation we used a construct with the PRM promoter producing GFP.
| cI autoinducing loop (BBa_K00) after 4 hours of incubation | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| File:File:cI4.png |
| Cel 6 | ||||||||||||||||
| Cel 7 | Cel 8 | Cel 9 | ||||||||||||||||
