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Team:Groningen/project characterisation promoters pbad
From 2011.igem.org
pBAD/AraC promoter
In our system and measurements we have been using the pBAD/AraC promoter ([http://partsregistry.org/Part:BBa_I0500 BBa_I0500]). This promoter is functioning in our circuit as a jammer for the LasR autoinducing loop. It is also used to induce the cI autoinducing loops during the measurements. That is why, in our opinion, characterizing this promoter in a way that would show us the activity rate not only depending on concentration of arabinose, but also in a dynamic way - over time - is crucial. The measurements were done as described in protocol.
Below you will find four figures representing the results of our measurements.
 Figure 1a. Cells carrying a pSB1C3 plasmid with pBAD/AraC-GFP-DT construct, induced at time=0h. |
 Figure 1b. Wild type cells, induced at time=0h. |
 Figure 2a. Cells carrying a pSB1C3 plasmid with pBAD/AraC-GFP-DT construct, induced at time=0h. |
 Figure 2b. Wild type cells, induced at time=0h. |
