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Team:Cornell/Week 16
From 2011.igem.org
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September 18th - September 24th
Sunday, September 18
Monday, September 19
Morning lab work done by: Jim Mathew
- ...
Afternoon-1 lab work done by: Youjin Cho
- ...
Afternoon-2 lab work done by: Charlie Chung
- Background
- The PCR site-directed mutagenesis done earlier today should ideally enhance protein expression of VioA, VioB, VioE, and RFP
- How?
- - By deleting excessive nucleotides between the ribosome binding site and the ATG start codon in the original sequences
- Work Accomplished
- [6:10pm] Plated the transformed products of VioA, VioB, VioE, and RFP on agar plates treated with ampicillin or carbenicillin (two are on amp; other two are on carb)
- Incubating at 37°C in Olin 303
