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Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs) | 
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We conducted a heat cycle PCR to determine the optimum conditions for amplifying our araC gene. The lanes here are loaded as follows:
1: 1kb+ ladder
2: Negative control (H2O)
3-7: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.
8: Negative control (H2O)
9-13: pBAD33 plasmid at temperatures 50C, 55C, 58C, 60C and 65C.
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