Team:Washington/Magnetosomes/GibsonVectors

From 2011.igem.org

DISCUSS GIBSON VECTORS AND WHY pGBs RULE AND pSB DROOL

Outline; What is gibson (and diagram from the slide)

New vectors... a diagram a table showing the antibiotics, copy number

This is an outline!!

What happened last year?

The Gibson Cloning method is definitely not a new terminology to the iGEM community. This cloning method waspublished in Nature protocols 2009 and it has gained a lot of attention from the iGEM community since then. The main reason behind is that it allows multiple inserts in one time isothermal reaction during the cloning process, which does not only saves a lot of time and efforts, but also allows more complexity when building the circuit.

Last year, the UW team used Gibson cloning method in place of traditional Biobrick restriction/ligation cloning for their project. To further improve the method, a “gibson reaction compatible” prefix and suffix were also designed based on BglBrick standard to increase the cloning efficiency.

What about this year?

Seeing that this is such a great method to do cloning...we CONTINUE WITH THE INVESTIGATION! AND WE MADE/SUBMITTED MORE VECTORS that are compatible with Gibson cloning in a hope to ease everyone’s pain when doing cloning. (bring the iGEM to a differnt level of cloning)

Next level of iGEM project: more complex circuit.

And we decided to look at magnetosome